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tianquick midi purification kit  (tiangen biotech co)


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    tiangen biotech co tianquick midi purification kit
    Tianquick Midi Purification Kit, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 95/100, based on 458 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tianquick+midi+purification+kit/TIANquick+Midi+Purification+Kit/pmc12995870-55-9-13
    Average 95 stars, based on 458 article reviews
    tianquick midi purification kit - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Purification:

    Article Title: High-Fidelity Data Retrieval from Synthetic DNA Pools via Machine Learning Model.
    Article Snippet: Synthetic DNA offers significant benefits, remarkable information density and long-term stability for data storage.. The practical success of DNA data storage, however, depends on solving the challenge of selective retrieval-accessing specific data with high fidelity from complex DNA mixtures.. Here, we present a machine learning method to achieve high-fidelity, isothermal selective data retrieval from synthetic DNA pools.

    Article Title: Functional customization of peptide linkers in fusion proteins through multimodal deep learning approach
    Article Snippet: .. Then, the PCR product was purified using either the TIANquick Midi Purification Kit (Tiangen Biotech (Beijing) Co., Ltd. #DP204) or the SPARKeasy Gel/PCR Purification Kit (Shandong Sikejie Biotechnology Co., Ltd. #AE0101), and the concentration of the DNA was measured by the Nanodrop (Allsheng, Hangzhou Allsheng Instruments Co., Ltd., Hangzhou, China). .. Furthermore, the random library fragments and the vector fragment were assembled using the ClonExpress II One Step Cloning Kit (Vazyme Biotech Co., Ltd. #C112) and recombinant products were transformed into E. coli BL21 Star (DE3) Competent Cell, the original plasmid pUC19-nanobody-GS linker-sfGFP was also transfected to serve as a positive control for subsequent studies.

    Article Title: Identification and Multigene Phylogenetic Analysis Reveal Alternaria as the Primary Pathogen Causing European Plum ( Prunus domestica ) Brown Spot in Xinjiang, China
    Article Snippet: Each 25.0 μL PCR mixture contained 12.5 μL 2× Taq Plus MasterMix II (Sangon Biotech (Shanghai) Co., Ltd., Shanghai, China), 1.0 μL of each forward and reverse primer, 2.0 μL DNA template, and 8.5 μL ddH 2 O [ ]. .. The PCR amplification protocol consisted of an initial denaturation at 94 °C for 4 min, followed by 35 cycles of denaturation at 94 °C for 30 s, annealing at 55 °C (ITS, endoPG), 62 °C (Alt a 1) or 59 °C (SSU, LSU) for 30 s, and extension at 72 °C for 30 s (ITS, endoPG), 45 s (Alt a 1) or 60 s (SSU, LSU), with a final extension at 72 °C for 10 min. Amplicons were examined on 1.0% agarose gels and purified using the TIANquick Midi Purification Kit (Tiangen Biotech, Beijing, China), then submitted to Tsingke Biological Technology Co., Ltd. (Xi’an, China) for bidirectional Sanger sequencing. .. Resulting sequences were manually edited and trimmed using BioEdit version 7.0.0, queried against the NCBI nucleotide (nt) database using the BLAST+ BLASTn algorithm ( https://ftp.ncbi.nlm.nih.gov/blast/executables/blast+/LATEST/?referrer=grok.com , 3 September 2025), and then submitted to GenBank.

    Article Title: Synthesis of tunable copolymers of 3-hydroxybutyrate and 3-hydroxyvalerate by engineered Halomonas bluephagenesis and their characterizations
    Article Snippet: Then, the fragments for plasmid construction were generated via polymerase chain reaction (PCR) amplification by High-Fidelity DNA polymerase (New England Biolabs, USA) using the isolated plasmid template. .. The TIANquick Midi Purification Kit (Tiangen Biotech Co., Ltd., China) was used to purify the DNA after the gel electrophoresis separation. ..

    Article Title: Binding Mechanism of PsauPBP3 to Sex Pheromones in Peridroma saucia : Insights from Computational and Experimental Approaches.
    Article Snippet: The PCR product was ligated into the pLB-T vector (TIANGEN) and transformed into E. coli Top10 cells. pLB-T plasmids containing the PsauPBP3 sequence were extracted with the TIANprep Mini Plasmid Kit (TIANGEN) and then digested with NdeI and EcoRI restriction enzymes for 2–3 h at 37 ◦C. .. The digestion product was purified from the agarose gel using the TIANquick MiDi Purification Kit (TIANGEN), then ligated into the expression vector pET28a (Novagen, Darmstadt, Germany). ..

    Article Title: Binding Mechanism of PsauPBP3 to Sex Pheromones in Peridroma saucia : Insights from Computational and Experimental Approaches
    Article Snippet: The PCR product was ligated into the pLB-T vector (TIANGEN) and transformed into E. coli Top10 cells. pLB-T plasmids containing the PsauPBP3 sequence were extracted with the TIANprep Mini Plasmid Kit (TIANGEN) and then digested with NdeI and EcoRI restriction enzymes for 2–3 h at 37 °C. .. The digestion product was purified from the agarose gel using the TIANquick MiDi Purification Kit (TIANGEN), then ligated into the expression vector pET28a (Novagen, Darmstadt, Germany). ..

    Article Title: Functional customization of peptide linkers in fusion proteins through multimodal deep learning approach
    Article Snippet: .. Then, the PCR product was purified using either the TIANquick Midi Purification Kit (Tiangen Biotech (Beijing) Co., Ltd. #DP204) or the SPARKeasy Gel/PCR Purification Kit (Shandong Sikejie Biotechnology Co., Ltd. #AE0101), and the concentration of the DNA was measured by the Nanodrop (Allsheng, Hangzhou Allsheng Instruments Co., Ltd., Hangzhou, China). .. Furthermore, the random library fragments and the vector fragment were assembled using the ClonExpress II One Step Cloning Kit (Vazyme Biotech Co., Ltd. #C112) and recombinant products were transformed into E. coli BL21 Star (DE3) Competent Cell, the original plasmid pUC19-nanobody-GS linker-sfGFP was also transfected to serve as a positive control for subsequent studies.

    Article Title: Identification and Multigene Phylogenetic Analysis Reveal Alternaria as the Primary Pathogen Causing European Plum ( Prunus domestica ) Brown Spot in Xinjiang, China.
    Article Snippet: Each 25.0 μL PCR mixture contained 12.5 μL 2× Taq Plus MasterMix II (Sangon Biotech (Shanghai) Co., Ltd., Shanghai, China), 1.0 μL of each forward and reverse primer, 2.0 μL DNA template, and 8.5 μL ddH2O [38]. .. The PCR amplification protocol consisted of an initial denaturation at 94 ◦C for 4 min, followed by 35 cycles of denaturation at 94 ◦C for 30 s, annealing at 55 ◦C (ITS, endoPG), 62 ◦C (Alt a 1) or 59 ◦C (SSU, LSU) for 30 s, and extension at 72 ◦C for 30 s (ITS, endoPG), 45 s (Alt a 1) or 60 s (SSU, LSU), with a final extension at 72 ◦C for 10 min. Amplicons were examined on 1.0% agarose gels and purified using the TIANquick Midi Purification Kit (Tiangen Biotech, Beijing, China), then submitted to Tsingke Biological Technology Co., Ltd. (Xi’an, China) for bidirectional Sanger sequencing. .. Resulting sequences were manually edited and trimmed using BioEdit version 7.0.0, queried https://doi.org/10.3390/jof12010069 against the NCBI nucleotide (nt) database using the BLAST+ BLASTn algorithm (https: //ftp.ncbi.nlm.nih.gov/blast/executables/blast+/LATEST/?referrer=grok.com, 3 September 2025), and then submitted to GenBank.

    Synthesized:

    Article Title: High-Fidelity Data Retrieval from Synthetic DNA Pools via Machine Learning Model.
    Article Snippet: Synthetic DNA offers significant benefits, remarkable information density and long-term stability for data storage.. The practical success of DNA data storage, however, depends on solving the challenge of selective retrieval-accessing specific data with high fidelity from complex DNA mixtures.. Here, we present a machine learning method to achieve high-fidelity, isothermal selective data retrieval from synthetic DNA pools.

    Polymerase Chain Reaction:

    Article Title: Functional customization of peptide linkers in fusion proteins through multimodal deep learning approach
    Article Snippet: .. Then, the PCR product was purified using either the TIANquick Midi Purification Kit (Tiangen Biotech (Beijing) Co., Ltd. #DP204) or the SPARKeasy Gel/PCR Purification Kit (Shandong Sikejie Biotechnology Co., Ltd. #AE0101), and the concentration of the DNA was measured by the Nanodrop (Allsheng, Hangzhou Allsheng Instruments Co., Ltd., Hangzhou, China). .. Furthermore, the random library fragments and the vector fragment were assembled using the ClonExpress II One Step Cloning Kit (Vazyme Biotech Co., Ltd. #C112) and recombinant products were transformed into E. coli BL21 Star (DE3) Competent Cell, the original plasmid pUC19-nanobody-GS linker-sfGFP was also transfected to serve as a positive control for subsequent studies.

    Article Title: Identification and Multigene Phylogenetic Analysis Reveal Alternaria as the Primary Pathogen Causing European Plum ( Prunus domestica ) Brown Spot in Xinjiang, China
    Article Snippet: Each 25.0 μL PCR mixture contained 12.5 μL 2× Taq Plus MasterMix II (Sangon Biotech (Shanghai) Co., Ltd., Shanghai, China), 1.0 μL of each forward and reverse primer, 2.0 μL DNA template, and 8.5 μL ddH 2 O [ ]. .. The PCR amplification protocol consisted of an initial denaturation at 94 °C for 4 min, followed by 35 cycles of denaturation at 94 °C for 30 s, annealing at 55 °C (ITS, endoPG), 62 °C (Alt a 1) or 59 °C (SSU, LSU) for 30 s, and extension at 72 °C for 30 s (ITS, endoPG), 45 s (Alt a 1) or 60 s (SSU, LSU), with a final extension at 72 °C for 10 min. Amplicons were examined on 1.0% agarose gels and purified using the TIANquick Midi Purification Kit (Tiangen Biotech, Beijing, China), then submitted to Tsingke Biological Technology Co., Ltd. (Xi’an, China) for bidirectional Sanger sequencing. .. Resulting sequences were manually edited and trimmed using BioEdit version 7.0.0, queried against the NCBI nucleotide (nt) database using the BLAST+ BLASTn algorithm ( https://ftp.ncbi.nlm.nih.gov/blast/executables/blast+/LATEST/?referrer=grok.com , 3 September 2025), and then submitted to GenBank.

    Article Title: Functional customization of peptide linkers in fusion proteins through multimodal deep learning approach
    Article Snippet: .. Then, the PCR product was purified using either the TIANquick Midi Purification Kit (Tiangen Biotech (Beijing) Co., Ltd. #DP204) or the SPARKeasy Gel/PCR Purification Kit (Shandong Sikejie Biotechnology Co., Ltd. #AE0101), and the concentration of the DNA was measured by the Nanodrop (Allsheng, Hangzhou Allsheng Instruments Co., Ltd., Hangzhou, China). .. Furthermore, the random library fragments and the vector fragment were assembled using the ClonExpress II One Step Cloning Kit (Vazyme Biotech Co., Ltd. #C112) and recombinant products were transformed into E. coli BL21 Star (DE3) Competent Cell, the original plasmid pUC19-nanobody-GS linker-sfGFP was also transfected to serve as a positive control for subsequent studies.

    Article Title: Identification and Multigene Phylogenetic Analysis Reveal Alternaria as the Primary Pathogen Causing European Plum ( Prunus domestica ) Brown Spot in Xinjiang, China.
    Article Snippet: Each 25.0 μL PCR mixture contained 12.5 μL 2× Taq Plus MasterMix II (Sangon Biotech (Shanghai) Co., Ltd., Shanghai, China), 1.0 μL of each forward and reverse primer, 2.0 μL DNA template, and 8.5 μL ddH2O [38]. .. The PCR amplification protocol consisted of an initial denaturation at 94 ◦C for 4 min, followed by 35 cycles of denaturation at 94 ◦C for 30 s, annealing at 55 ◦C (ITS, endoPG), 62 ◦C (Alt a 1) or 59 ◦C (SSU, LSU) for 30 s, and extension at 72 ◦C for 30 s (ITS, endoPG), 45 s (Alt a 1) or 60 s (SSU, LSU), with a final extension at 72 ◦C for 10 min. Amplicons were examined on 1.0% agarose gels and purified using the TIANquick Midi Purification Kit (Tiangen Biotech, Beijing, China), then submitted to Tsingke Biological Technology Co., Ltd. (Xi’an, China) for bidirectional Sanger sequencing. .. Resulting sequences were manually edited and trimmed using BioEdit version 7.0.0, queried https://doi.org/10.3390/jof12010069 against the NCBI nucleotide (nt) database using the BLAST+ BLASTn algorithm (https: //ftp.ncbi.nlm.nih.gov/blast/executables/blast+/LATEST/?referrer=grok.com, 3 September 2025), and then submitted to GenBank.

    Concentration Assay:

    Article Title: Functional customization of peptide linkers in fusion proteins through multimodal deep learning approach
    Article Snippet: .. Then, the PCR product was purified using either the TIANquick Midi Purification Kit (Tiangen Biotech (Beijing) Co., Ltd. #DP204) or the SPARKeasy Gel/PCR Purification Kit (Shandong Sikejie Biotechnology Co., Ltd. #AE0101), and the concentration of the DNA was measured by the Nanodrop (Allsheng, Hangzhou Allsheng Instruments Co., Ltd., Hangzhou, China). .. Furthermore, the random library fragments and the vector fragment were assembled using the ClonExpress II One Step Cloning Kit (Vazyme Biotech Co., Ltd. #C112) and recombinant products were transformed into E. coli BL21 Star (DE3) Competent Cell, the original plasmid pUC19-nanobody-GS linker-sfGFP was also transfected to serve as a positive control for subsequent studies.

    Article Title: Functional customization of peptide linkers in fusion proteins through multimodal deep learning approach
    Article Snippet: .. Then, the PCR product was purified using either the TIANquick Midi Purification Kit (Tiangen Biotech (Beijing) Co., Ltd. #DP204) or the SPARKeasy Gel/PCR Purification Kit (Shandong Sikejie Biotechnology Co., Ltd. #AE0101), and the concentration of the DNA was measured by the Nanodrop (Allsheng, Hangzhou Allsheng Instruments Co., Ltd., Hangzhou, China). .. Furthermore, the random library fragments and the vector fragment were assembled using the ClonExpress II One Step Cloning Kit (Vazyme Biotech Co., Ltd. #C112) and recombinant products were transformed into E. coli BL21 Star (DE3) Competent Cell, the original plasmid pUC19-nanobody-GS linker-sfGFP was also transfected to serve as a positive control for subsequent studies.

    Amplification:

    Article Title: Identification and Multigene Phylogenetic Analysis Reveal Alternaria as the Primary Pathogen Causing European Plum ( Prunus domestica ) Brown Spot in Xinjiang, China
    Article Snippet: Each 25.0 μL PCR mixture contained 12.5 μL 2× Taq Plus MasterMix II (Sangon Biotech (Shanghai) Co., Ltd., Shanghai, China), 1.0 μL of each forward and reverse primer, 2.0 μL DNA template, and 8.5 μL ddH 2 O [ ]. .. The PCR amplification protocol consisted of an initial denaturation at 94 °C for 4 min, followed by 35 cycles of denaturation at 94 °C for 30 s, annealing at 55 °C (ITS, endoPG), 62 °C (Alt a 1) or 59 °C (SSU, LSU) for 30 s, and extension at 72 °C for 30 s (ITS, endoPG), 45 s (Alt a 1) or 60 s (SSU, LSU), with a final extension at 72 °C for 10 min. Amplicons were examined on 1.0% agarose gels and purified using the TIANquick Midi Purification Kit (Tiangen Biotech, Beijing, China), then submitted to Tsingke Biological Technology Co., Ltd. (Xi’an, China) for bidirectional Sanger sequencing. .. Resulting sequences were manually edited and trimmed using BioEdit version 7.0.0, queried against the NCBI nucleotide (nt) database using the BLAST+ BLASTn algorithm ( https://ftp.ncbi.nlm.nih.gov/blast/executables/blast+/LATEST/?referrer=grok.com , 3 September 2025), and then submitted to GenBank.

    Article Title: Identification and Multigene Phylogenetic Analysis Reveal Alternaria as the Primary Pathogen Causing European Plum ( Prunus domestica ) Brown Spot in Xinjiang, China.
    Article Snippet: Each 25.0 μL PCR mixture contained 12.5 μL 2× Taq Plus MasterMix II (Sangon Biotech (Shanghai) Co., Ltd., Shanghai, China), 1.0 μL of each forward and reverse primer, 2.0 μL DNA template, and 8.5 μL ddH2O [38]. .. The PCR amplification protocol consisted of an initial denaturation at 94 ◦C for 4 min, followed by 35 cycles of denaturation at 94 ◦C for 30 s, annealing at 55 ◦C (ITS, endoPG), 62 ◦C (Alt a 1) or 59 ◦C (SSU, LSU) for 30 s, and extension at 72 ◦C for 30 s (ITS, endoPG), 45 s (Alt a 1) or 60 s (SSU, LSU), with a final extension at 72 ◦C for 10 min. Amplicons were examined on 1.0% agarose gels and purified using the TIANquick Midi Purification Kit (Tiangen Biotech, Beijing, China), then submitted to Tsingke Biological Technology Co., Ltd. (Xi’an, China) for bidirectional Sanger sequencing. .. Resulting sequences were manually edited and trimmed using BioEdit version 7.0.0, queried https://doi.org/10.3390/jof12010069 against the NCBI nucleotide (nt) database using the BLAST+ BLASTn algorithm (https: //ftp.ncbi.nlm.nih.gov/blast/executables/blast+/LATEST/?referrer=grok.com, 3 September 2025), and then submitted to GenBank.

    Sequencing:

    Article Title: Identification and Multigene Phylogenetic Analysis Reveal Alternaria as the Primary Pathogen Causing European Plum ( Prunus domestica ) Brown Spot in Xinjiang, China
    Article Snippet: Each 25.0 μL PCR mixture contained 12.5 μL 2× Taq Plus MasterMix II (Sangon Biotech (Shanghai) Co., Ltd., Shanghai, China), 1.0 μL of each forward and reverse primer, 2.0 μL DNA template, and 8.5 μL ddH 2 O [ ]. .. The PCR amplification protocol consisted of an initial denaturation at 94 °C for 4 min, followed by 35 cycles of denaturation at 94 °C for 30 s, annealing at 55 °C (ITS, endoPG), 62 °C (Alt a 1) or 59 °C (SSU, LSU) for 30 s, and extension at 72 °C for 30 s (ITS, endoPG), 45 s (Alt a 1) or 60 s (SSU, LSU), with a final extension at 72 °C for 10 min. Amplicons were examined on 1.0% agarose gels and purified using the TIANquick Midi Purification Kit (Tiangen Biotech, Beijing, China), then submitted to Tsingke Biological Technology Co., Ltd. (Xi’an, China) for bidirectional Sanger sequencing. .. Resulting sequences were manually edited and trimmed using BioEdit version 7.0.0, queried against the NCBI nucleotide (nt) database using the BLAST+ BLASTn algorithm ( https://ftp.ncbi.nlm.nih.gov/blast/executables/blast+/LATEST/?referrer=grok.com , 3 September 2025), and then submitted to GenBank.

    Article Title: Identification and Multigene Phylogenetic Analysis Reveal Alternaria as the Primary Pathogen Causing European Plum ( Prunus domestica ) Brown Spot in Xinjiang, China.
    Article Snippet: Each 25.0 μL PCR mixture contained 12.5 μL 2× Taq Plus MasterMix II (Sangon Biotech (Shanghai) Co., Ltd., Shanghai, China), 1.0 μL of each forward and reverse primer, 2.0 μL DNA template, and 8.5 μL ddH2O [38]. .. The PCR amplification protocol consisted of an initial denaturation at 94 ◦C for 4 min, followed by 35 cycles of denaturation at 94 ◦C for 30 s, annealing at 55 ◦C (ITS, endoPG), 62 ◦C (Alt a 1) or 59 ◦C (SSU, LSU) for 30 s, and extension at 72 ◦C for 30 s (ITS, endoPG), 45 s (Alt a 1) or 60 s (SSU, LSU), with a final extension at 72 ◦C for 10 min. Amplicons were examined on 1.0% agarose gels and purified using the TIANquick Midi Purification Kit (Tiangen Biotech, Beijing, China), then submitted to Tsingke Biological Technology Co., Ltd. (Xi’an, China) for bidirectional Sanger sequencing. .. Resulting sequences were manually edited and trimmed using BioEdit version 7.0.0, queried https://doi.org/10.3390/jof12010069 against the NCBI nucleotide (nt) database using the BLAST+ BLASTn algorithm (https: //ftp.ncbi.nlm.nih.gov/blast/executables/blast+/LATEST/?referrer=grok.com, 3 September 2025), and then submitted to GenBank.

    Nucleic Acid Electrophoresis:

    Article Title: Synthesis of tunable copolymers of 3-hydroxybutyrate and 3-hydroxyvalerate by engineered Halomonas bluephagenesis and their characterizations
    Article Snippet: Then, the fragments for plasmid construction were generated via polymerase chain reaction (PCR) amplification by High-Fidelity DNA polymerase (New England Biolabs, USA) using the isolated plasmid template. .. The TIANquick Midi Purification Kit (Tiangen Biotech Co., Ltd., China) was used to purify the DNA after the gel electrophoresis separation. ..

    Agarose Gel Electrophoresis:

    Article Title: Binding Mechanism of PsauPBP3 to Sex Pheromones in Peridroma saucia : Insights from Computational and Experimental Approaches.
    Article Snippet: The PCR product was ligated into the pLB-T vector (TIANGEN) and transformed into E. coli Top10 cells. pLB-T plasmids containing the PsauPBP3 sequence were extracted with the TIANprep Mini Plasmid Kit (TIANGEN) and then digested with NdeI and EcoRI restriction enzymes for 2–3 h at 37 ◦C. .. The digestion product was purified from the agarose gel using the TIANquick MiDi Purification Kit (TIANGEN), then ligated into the expression vector pET28a (Novagen, Darmstadt, Germany). ..

    Article Title: Binding Mechanism of PsauPBP3 to Sex Pheromones in Peridroma saucia : Insights from Computational and Experimental Approaches
    Article Snippet: The PCR product was ligated into the pLB-T vector (TIANGEN) and transformed into E. coli Top10 cells. pLB-T plasmids containing the PsauPBP3 sequence were extracted with the TIANprep Mini Plasmid Kit (TIANGEN) and then digested with NdeI and EcoRI restriction enzymes for 2–3 h at 37 °C. .. The digestion product was purified from the agarose gel using the TIANquick MiDi Purification Kit (TIANGEN), then ligated into the expression vector pET28a (Novagen, Darmstadt, Germany). ..

    Expressing:

    Article Title: Binding Mechanism of PsauPBP3 to Sex Pheromones in Peridroma saucia : Insights from Computational and Experimental Approaches.
    Article Snippet: The PCR product was ligated into the pLB-T vector (TIANGEN) and transformed into E. coli Top10 cells. pLB-T plasmids containing the PsauPBP3 sequence were extracted with the TIANprep Mini Plasmid Kit (TIANGEN) and then digested with NdeI and EcoRI restriction enzymes for 2–3 h at 37 ◦C. .. The digestion product was purified from the agarose gel using the TIANquick MiDi Purification Kit (TIANGEN), then ligated into the expression vector pET28a (Novagen, Darmstadt, Germany). ..

    Article Title: Binding Mechanism of PsauPBP3 to Sex Pheromones in Peridroma saucia : Insights from Computational and Experimental Approaches
    Article Snippet: The PCR product was ligated into the pLB-T vector (TIANGEN) and transformed into E. coli Top10 cells. pLB-T plasmids containing the PsauPBP3 sequence were extracted with the TIANprep Mini Plasmid Kit (TIANGEN) and then digested with NdeI and EcoRI restriction enzymes for 2–3 h at 37 °C. .. The digestion product was purified from the agarose gel using the TIANquick MiDi Purification Kit (TIANGEN), then ligated into the expression vector pET28a (Novagen, Darmstadt, Germany). ..



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